Chromomycin-a3
WebNov 19, 1985 · The binding of chromomycin A3 to calf thymus DNA and poly (dG-dC) has been studied by 13C and 1H NMR with emphasis on the mode of binding, the role of Mg2+, and pH effects. WebChromomycin A 3 and mithramycin are antibiotics binding preferentially to the GC-rich regions of DNA. They have excitation maxima at 440 nm and emission maxima at 555 …
Chromomycin-a3
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WebChromomycin A3 445 575 CyTRAK Orange™* 457,488,549 615 Ethidium Bromide 493 620 Propidium iodide (PI) 305,540 620 325,360,488 DRAQ5™ 646 681,697 DRAQ7™ 599, 644 678,697 Single dye F ora singl efu c t dy, hx w vp electromagnetic energy to an electron in that molecule (1). The electron moves to an excitation state at the next energy level ... Web(Hoechst 33258, Chromomycin A3). Highly purified fractions of chromosomes can then be isolated rapidly from the labeled chromosomes using the MoFlo High performance Cell Sorter. Chromosome preparation and staining. Block cells at metaphase using 0.1 µg/ml demecolcine for an optimized amount of time to maximize the percentage of mitotic cells
Web其显示的人类染色体带型与Q带或G带相反,异染色质区如染色体的,一般在Q带或G显带中深染, 常染色质 区浅染,而R带则异染色质区一般浅染,常染色质区深染。. 其他R显带的方法包括用chromomycin A3或olivomycin等荧光素染色,或在 细胞周期 的合成期(S期)将 ... WebApplication: Chromomycin A3 is a G-C specific DNA ligand which inhibits transcription and acts as a DNA binding dye CAS Number: 7059-24-7 Purity: ≥97% Molecular Weight: 1183.25 Molecular Formula: C 57 H 82 …
WebG-C specific intercalating agents [actinomycin D (AMD) and chromomycin A3 at 1 microM] also prevented ER disappearance; ethidium bromide (EB) and quinacrine were ineffective. AMD and CHX operated immediately after their addition to the medium indicating an inhibitory action on the synthesis of an RNA and/or a peptide with high turnover rate ... WebApr 27, 2024 · Chromomycin A3 (which is interesting also an antibiotic!), which binds to GC-rich regions of DNA. A Hoechst stain (e.g. 33258 or 33342), that binds to AT-rich regions. Using these DNA stains, we can separate the larger chromosomes from the smaller ones as they will contain more DNA and will thus yield a greater fluorescence signal.
WebBiosynthesis of mithramycin, chromomycin A3, and their derivatives has been investigated in detail . In addition to their antiproliferative potency, AA antibiotics demonstrate an antiviral activity [9,10]. Mithramycin has been tested as a neuroprotector for Huntington’s disease and neuronal death [11,12].
WebDec 1, 2014 · Chromomycin A3 suppresses cholangiocarcinoma growth by induction of S phase cell cycle arrest and suppression of Sp1-related anti-apoptotic proteins Paksiree Saranaruk, R. Kariya, +7 authors K. Vaeteewoottacharn Biology, Chemistry International journal of molecular medicine 2024 TLDR switch iphone 13 to lteWebFawn Creek Township is a locality in Kansas. Fawn Creek Township is situated nearby to the village Dearing and the hamlet Jefferson. Map. Directions. Satellite. Photo Map. switch iphone sim cards to transfer phoneWebDec 4, 2014 · The present study highlights the biological effects of chromomycin A2 toward metastatic melanoma cells in culture. Besides chromomycin A2, chromomycin A3 and demethylchromomycin A2 were also identified from the extract derived from Streptomyces sp., recovered from Paracuru Beach, located in the northeast region of Brazil. The … switchipidoWebApr 6, 2024 · Chromomycin A3 (CMA3, Figure 17.1) is an antibiotic glycoside produced by the fermentation of a certain strain of Streptomyces griseous. In the presence of bivalent … switch iphone to iphoneWebChromomycin A₃ - CAS 7059-24-7 - Calbiochem Antitumor antibiotic that inhibits RNA synthesis. A cell-permeable fluorescent dye that can be used with Bisbenzimide H 33258 … switch ipad輸出WebChromomycin A 3 is an anthraquinone antibiotic and antitumor agent isolated from S. griseus that is used as a fluorescent probe for DNA with excitation/emission spectra of 445/575 nm. 1, 2 Its DNA binding is specific to two or more contiguous GC base pairs, which makes it suitable for characterizing heterochromatin in plants with species ... switch ipbanWebJun 2, 2024 · protamine deficiency by chromomycin A3 (CMA3) staining. In addition, the relationship between the fertilisation rate and sperm ubiquitination was calculated in ICSI candidates. The intensity of ubiquitination showed a significant negative correlation with sperm concentration (r = 0.255, P = 0.032) and a switch iphone from 5g to 4g