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Peasy®-blunt e1 expression vector

WebNov 15, 2024 · Global-transcriptome analysis of all wild tree peony species and 60 cultivars revealed five candidate genes that may be involved in key steps of linalool biosynthesis, especially the expressions of... WebNov 7, 2024 · The sequenced cDNA of six candidate PdTPS genes was amplified and subcloned into the pEASY ® -Blunt E1 Expression Vector (TransGen) to express proteins in E. coli strain Transetta (DE3). Liquid cultures of the bacteria harboring the empty vector and different PdTPS genes were grown at 37°C to an OD 600 of 0.6.

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WebMay 1, 2024 · Expression analysis of the BoaZDS gene in E. coli The BoaZDS fragment was inserted into the pEASY-Blunt E1 vector, and a recombinant prokaryotic expression vector was constructed. The recombinant plasmid pEASY -Blunt E1- BoaZDS was then transformed into E. coli Transetta (DE3) cells. WebProduct Details. pEASY® -Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. … shower tile floor installation https://laurrakamadre.com

pEASY-Blunt Simple (linearized) Sequence and Map - SnapGene

WebNov 19, 2016 · The pEASY-Blunt E1 plasmid (TransGen Biotech, China) was used as the cloning vector and expression vector. The E. coli strains were cultivated in Luria-Bertani (LB) medium containing ampicillin (100 µg/ml) when necessary. Construction of recombinant plasmid The full sequence of TrXyn10 was amplified from T. rubra YIM 77501 T genomic … WebAug 28, 2024 · Finally, the prokaryotic expression vector pEASY ® -Blunt E1-IrUGT86A1 was successfully used to express about 53 KD of IrUGT86A1-like protein. This research builds a foundation for further investigation on the function of this gene in the synthesis and modification of secondary metabolites. View Full-Text WebpEASY ®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient … shower tile floor ideas

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Category:Biogenesis of flavor-related linalool is diverged and genetically ...

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Peasy®-blunt e1 expression vector

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WebpEASY-Blunt Simple (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter but no … WebJun 2, 2024 · Briefly, the PCR products of target genes (without signal peptides) were purified and then cloned into a pEASY-Blunt E1 expression vector (TransGen). E. coli BL21 (DE3) cells containing the expression vector were grown at 37 °C to an OD600 of ~0.8 and induced with isopropyl-β-D-thiogalactopyranoside (IPTG) at a final concentration of 1 mM …

Peasy®-blunt e1 expression vector

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WebSep 30, 2024 · (PDF) Effective Expression of the Serratia marcescens Phospholipase A1 Gene in Escherichia coli BL21 (DE3), Enzyme Characterization, and Crude Rapeseed Oil Degumming via a Free Enzyme Approach WebApr 15, 2024 · The E. coli expression vector pEASY-Blunt E1-BvGSTU9 (TransGen) was generated by amplifying BvGSTU9 using the primers BvGSTU9-F (5’ …

Web本发明涉及生物技术，具体的是一种乙酰海藻酸钠酯酶及该酯酶在降低铜绿假单胞杆菌frd1耐药性方面的应用。乙酰海藻酸钠酯酶如序列表seq id no:1中碱基所示。所述乙酰海藻酸钠酯酶在降解乙酰化海藻酸钠中的应用。所述乙酰海藻酸钠酯酶在防治铜绿假单胞杆菌感染中的应用 … WebPeasy Blunt E1 Expression Vector, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, …

Web糙皮侧耳新831菌株为河南农业大学生命科学学院应用真菌研究室保藏；pEASY-blunt E1 expression kit、pEASY-blunt cloning kit、TransStart FastPfu DNA polymerase购自北京全式金生物技术有限公司；E.coliDH5α、E.coliBL21（DE3）购自北京博迈德基因技术有限公司；RNA trizol plus购自大连宝 ... WebThen msv was cloned into the prokaryotic expression vector pEASY -Blunt E1. The fusion protein msv was expressed by pEASY -Blunt E1 under the induction of isopropyl-β-d …

WebFree Trial pEASY-Blunt E1 (linearized) Prokaryotic expression vector for cloning blunt-ended PCR products and adding an N-terminal 6xHis tag. Sequence Author: TransBionovo …

WebMay 1, 2024 · The recombinant plasmid pEASY-Blunt E1-BoaZDS was then transformed into E. coli Transetta (DE3) cells. Upon reaching an OD 600 of 0.6–0.8, expression in the … shower tile for floorWebOct 15, 2024 · Expression of boaugt74b1 in escherichia coli. UGT74B1 CDS was ligated with the pEASY-Blunt E1 expression vector and transformed into Trans1-T1 (TransGene, … shower tile floor sealerWebpEASY-Blunt E2 (linearized) Prokaryotic expression vector for cloning blunt-ended PCR products and adding a C-terminal 6xHis tag. Sequence Author: TransBionovo (TransGen … shower tile for small showershower tile for shampooWebMay 26, 2024 · Using the TIANprep miniature plasmid kit (Tiangen, Beijing, China), the plasmid DNA were isolated from the recombinant clone, and sequencing confirmed the insert pEASY-Blunt no.e1-YtnP. Next, the recombinant plasmid pEASY Blunt E1-YtnP into E. coli BL21 (DE3) cells, 37 °C in containing 50 mg/mL of ampicillin LB culture medium for … shower tile grout repair servicesWebFeb 28, 2024 · The results of semi-reverse transcription-PCR and real-time PCR analysis verified BjHMA4 expression in the transgenic rice and wheat (Additional file 1: Data S1). The overexpression of BjHMA4 in rice and wheat, which was driven by the ubiquitin and Actin1 promoter respectively, affected the Zn/Cd distribution of the transgenic plants. shower tile envelope cut floorWebJan 15, 2024 · In Pseudomonas aeruginosa PAO1, exogenous AI-2 influenced the biofilm amount and virulence factor expression in a dose-dependent manner in vitro and increased the histological lung damage in mice. 9 In Streptococcus suis, AI-2 supplemented exogenously had an impact on the expression of virulence genes and host–cell … shower tile grout thickness