Psmart bac
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Psmart bac
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WebDec 9, 2024 · The pSMART BAC HindIII vector (7.6 kb) was fully digested and dephosphorylated by using HindIII restriction enzyme and calf intestinal alkaline phosphatase (CIAP) enzyme (NEB, UK) at 37 °C for 60 min. Afterwards, the partially digested oral metagenome was ligated into a pre-digested pSMART BAC vector by using Anza T4 … WebInsignia™ - 43" Class F30 Series LED 4K UHD Smart Fire TV. 4.6(2,020) $299.99.
WebSUNY Upstate Medical. MedPrep Academic Enhancement One-Year MS Program. Master's in Public Health (MPH) Medical Scholars Program. The Medical Scholars Master's in … Webin BAC cloning and library construction. ˛ e pSMART® BAC or pEZ BAC vectors incorporate the CloneSmart® transcription-free technology to minimize cloning bias and enable …
WebFree Trial. pSMART BAC v2.0. BAC vector with inducible copy number, suitable for cloning toxic inserts. Sequence Author: Lucigen. Open in SnapGene. Try SnapGene for Free. … WebpSMART ® BAC V2.0 cloning vector. 1,2,3 The spike gene was replaced by a (S) luciferase (Luc2) and enhanced green fluorescent protein (eGFP) fusion construct. The Luc2 gene is …
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WebpSMART BAC v2.0 (BamHI linearized) Sequence and Map. BamHI-linearized and dephosphorylated BAC vector with inducible copy number, suitable for cloning toxic … tasp cert armyWebpSmartBAC-S.gbk A portion of this plasmid was derived from a plasmid made by The gene cassette was synthesized by Dr. ChengCang Wu, formerly of Lucigen. More information if … tas performance warehouse hobartWeb2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g. 5.Store the plasmid at -20 ℃. pSMART BAC v2.0 vector Sequence Copy Sequence tas petroleum account loginWebHours. Belle Glade Testing Monday - Thursday: 8am - 7pm Friday (Fall & Spring): 8am - 12pm Friday (Summer): Closed. Boca Raton Testing Monday - Thursday: 8am - 7pm tas performance solingenWebThe pSMART® BAC vectors incorporate transcription-free cloning to provide maximum stability. These vectors are supplied pre-cut at BamHI, EcoRI, or HindIII sites, with dephosphorylated ends. The pSMART BAC vectors have a lacZ-sacB stuffer region, allowing uncut vector to be detected by blue/white screening and selected against by plating on ... tasp fort carsonWebA bacterial artificial chromosome ( BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid ), used for transforming and cloning in bacteria, usually E. coli. [1] [2] [3] F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division. the bulls head coalvilleWebApr 29, 2015 · To develop a fungal artificial chromosome (FAC) system, we used unbiased Random Shear BACs as the basis for our technology as BAC inserts can reach up to 300 kb, and Random Shear BAC cloning generates even coverage of a fungal genome for the selection of all SM clusters within the genome [ 21, 23 ]. tasp hammond la