Tr-fret fxr coactivator recruitment assay
WebThe most common lanthanides used in TR-FRET assays for HTS are terbium and europium. Terbium offers unique advantages over europium when used as the donor species in a TR …
Tr-fret fxr coactivator recruitment assay
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WebNov 3, 2024 · The LanthaScreen TR-FRET FXR (Farnesoid X Receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled a WebCatalog number: A15129. The LanthaScreen™ TR-FRET LXR (liver X receptor) beta Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR beta ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR ...
WebThe results are in agreement with the results from the TR-FRET coactivator recruitment assays, where MRL-871 and Bit-L15 show a similar overall affinity for RORγt, while MRL-L15 is less potent ... WebCatalog number: PV4833. The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput …
WebMay 1, 2007 · To evaluate if T0901317 binds PXR directly, we used a cell-free FRET-based coactivator recruitment assay. T0901317 induced recruitment of a peptide derived from SRC-1 to the LBD of hPXR (EC 50 = 27 nM) with the same efficacy of as rifampicin, a bona fide hPXR ligand (EC 50 = 2 μM). WebThe FXR (Farnesoid X Receptor) Agonist Assay Kit (TR-FRET) is designed to measure binding of the FXR and SRC-1 (Steroid Receptor Coactivator 1) for screening FXR agonists …
WebHowever, there has been only limited progress in using this assay format for pregnane X receptor (PXR). In this chapter, we discuss TR-FRET protein-protein interaction assays and focus on a novel PXR TR-FRET coactivator interaction assay that we have developed based on a PXR coactivator cocrystal study.
WebWith the introduction of Terbium (Tb(3+))-based time-resolved fluorescence energy transfer (TR-FRET), it has become possible to measure 2 different interactions within 1 test tube in parallel. The authors have applied this technology to develop a dual readout system for the simultaneous monitoring of steroid hormone site binding and cofactor peptide recruitment. projected screenWebHere, we describe a TR-FRET assay that monitors the interaction of the estrogen receptor (ER) α ligand binding domain (labeled with a terbium chelate via a streptavidin-biotin interaction) with a sequence of coactivator protein SRC3 (labeled directly with fluorescein) and the disruption of this interaction with a peptide and a small molecule ... lab grown diamonds cincinnatiWebMay 7, 2013 · FXR TR-FRET assay [19, 20] A 20-lL ... to alter CDCA-mediated FXR binding using a panel of 154 coregulator motifs and to induce or inhibit transactivation and coactivator recruitment activities of ... lab grown diamonds businessWebDescription. The LanthaScreen™ TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR … lab grown diamonds austinWebSep 26, 2014 · The FXR-bla assay is able to ... The antagonist assay was conducted in the presence of 50 μM CDCA to induce coactivator recruitment. The TR-FRET ratios were normalized using DMSO as 0% ... projected sea level 2100 mapWebFeb 15, 2024 · The TR-FRET is an in vitro ligand-induced FXR coactivator interaction assay, which measures the binding of compound to the FXR ligand binding domain (LBD) protein … lab grown diamonds costWebThe LanthaScreen TR-FRET FXR (farnesoid X receptor) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential FXR ligands as … projected sec tournament bracket